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While there have been some published papers regarding the Solfeggio Frequencies, there is still much research to be done. New research is on the way! Here are some papers that studied the Solfeggio Frequencies.

A 2018 study on 528hz frequencies and how the effect the endocrine system by Kaho Akimoto, Ailing Hu, and Takuji Yamaguchi showed that a group of people who listened to 528hz tones had decreased stress compared to a control group.

A paper done on the Effects of 528 Hz Sound Wave to Reduce Cell Death in Human Astrocyte Primary Cell Culture Treated with Ethanol found the use of these sound waves can be useful to reduce the toxic effects of ethanol on astrocytes cells culture.

Sonia Joseph at the American College of Healthcare Sciences has a magnificent paper demonstrating how Solfeggio Frequencies help heal DNA and trauma.

Lili Naghdi,  Heidi Ahonen, Pasqualino Macario, and  Lee Bartel have a clinical study showing the effect of low-frequency sound stimulation on patients with fibromyalgia.

Got it. I’ll conduct a comprehensive search for all available medical and scientific studies involving solfeggio frequencies (such as 174 Hz, 285 Hz, 396 Hz, etc.) and the 432 Hz tuning. This will include peer-reviewed papers, academic theses, pilot studies, and any relevant gray literature, across all health domains and in any language.

I’ll organize the results in a clear, structured format and highlight key findings from each study. I’ll let you know as soon as everything is ready.

Studies on 528 Hz (“Love” or “Miracle” Frequency)

Studies on 432 Hz (Alternative Tuning “Verdi’s A”)

  • Music Tuned to 440 Hz versus 432 Hz and the Health Effects: A Double-blind Cross-over Pilot Study (D. Calamassi, G.P. Pomponi – 2019). Frequency: 432 Hz vs. standard 440 Hz tuning. Objective: Determine if music tuned to 432 Hz produces different physiological and psychological effects than 440 Hz music. Methodology: 33 healthy volunteers underwent two listening sessions (20 minutes each) on separate days in a random order – one with music (instrumental soundtrack) tuned to 440 Hz and one tuned to 432 Hz. Vital signs (blood pressure, heart rate, respiratory rate, oxygen saturation) and self-reported feelings (physical/emotional sensations, concentration, satisfaction) were recorded after each session. Key Findings: Music at 432 Hz tended to lower blood pressure (both systolic/diastolic) slightly more than 440 Hz (non-significant) and produced a significant reduction in heart rate (~5 bpm lower on average, p≈0.05). Respiratory rate was also mildly lower with 432 Hz (p≈0.06). Participants reported feeling more focused and more satisfied after listening to 432 Hz-tuned music compared to 440 Hz. Conclusion: 432 Hz music can induce slightly greater relaxation (marked by lower heart rate) and improved listener satisfaction; the authors suggest larger trials to further verify these effects.
  • Effect of Music at 432 Hz and 440 Hz on Dental Anxiety and Salivary Cortisol Levels in Patients Undergoing Tooth Extraction: A Randomized Clinical Trial (P.C. Aravena, C. Almonacid, M.I. Mancilla – 2020). Frequency: 432 Hz vs. 440 Hz vs. no music. Objective: Compare the anxiety-reducing effect of music tuned to 432 Hz versus 440 Hz (or silence) in a stressful clinical setting (dental surgery). Methodology: 42 dental patients with moderate baseline anxiety were randomized into three groups before a tooth extraction: one group listened to calming music tuned to 432 Hz (15 min), another listened to the same music tuned to 440 Hz (15 min), and a control group wore headphones with no music. Anxiety was measured by the Corah Dental Anxiety Scale, and stress was assessed via salivary cortisol, both before and after the music intervention. Key Findings: Both music groups experienced significantly lower anxiety scores post-intervention compared to the no-music control (mean anxiety ~8.5 in 432/440 Hz groups vs. 17.2 in controls, p<0.05). Notably, the 432 Hz group showed significantly lower cortisol levels after music (0.49 μg/dL) than both the 440 Hz group (1.35 μg/dL) and controls (1.59 μg/dL). In other words, while any music reduced patients’ anxiety, only 432 Hz-tuned music led to a marked drop in stress hormone (cortisol). Conclusion: Listening to music during dental procedures can alleviate anxiety, and 432 Hz tuning in particular may have a stronger stress-relieving biochemical effect (cortisol reduction) than the standard 440 Hz.
  • EEG Research of Music Impact Tuned on 432 Hz Pitch vs. 440 Hz (Branislav R. Tanasić – 2022). Frequency: 432 Hz vs. 440 Hz. Objective: Use electroencephalography (EEG) to directly compare brainwave responses and listener engagement when music is tuned to 432 Hz versus 440 Hz. Methodology: 74 individuals (33 female, 41 male) listened to a series of instrumental musical pieces—recorded with real instruments tuned to 432 Hz and again to 440 Hz—while undergoing EEG recording. The study ensured the musical selections were unfamiliar to avoid bias from personal music preference. EEG data (across multiple scalp electrodes) were analyzed for patterns indicating attention and emotional engagement (e.g. relative theta and beta wave power in frontal regions), and participants’ subjective preferences were also noted. Key Findings: Brainwave analysis indicated greater listener attention and emotional involvement during 432 Hz music. In particular, the ratio of theta to beta activity (an indicator of focused attention vs. relaxation) suggested enhanced focus with 432 Hz stimuli. Many participants showed a slight subjective preference for music at 432 Hz tuning as well. However, inter-individual variability was present, and the overall preference differences were modest. Conclusion: 432 Hz-tuned music tends to engage the brain’s attention and emotional centers slightly more than 440 Hz, potentially making it more soothing and immersive, though individual responses vary. The author notes that personal experience and melody familiarity can influence results, but overall there was a measurable neurophysiological bias toward 432 Hz.

Studies Involving Full Solfeggio Scale or Other Specific Tones

  • Effects of Solfeggio Frequency Exposure on DNA Structure (In Vitro) – Glen Rein, Jonathan Goldman (2024). Frequencies: Full set of 9 Solfeggio frequencies (174, 285, 396, 417, 528, 639, 741, 852, 963 Hz), tested sequentially; also 528 Hz alone. Objective: Assess whether Solfeggio tones influence physical properties of human DNA in solution. Methodology: In a controlled lab setting, purified human DNA (from placenta) suspended in water was placed in front of a speaker and exposed to sound recordings. Researchers tested two conditions: (1) a single 528 Hz tone (from a tuning fork recording) looped continuously, and (2) an ascending sequence of all nine Solfeggio frequencies played in order on tuning forks (174→963 Hz, looped). For comparison, a descending sequence 963→174 Hz was also tested. The primary outcome was DNA’s electrical conductivity (related to its helical structure and strand winding), measured before and after exposure. Key Findings: The 528 Hz single-tone by itself did not produce any significant change in DNA’s structural metrics. In contrast, the full Solfeggio frequency ascending sequence caused a dramatic change – a 79% decrease in DNA electrical conductivity (indicating a possible unwinding or conformational change in the double helix). Strikingly, when the same nine tones were played in descending order, the effect was minimal (~7% decrease). This suggests that playing the Solfeggio tones in increasing order has a unique, strong effect on DNA, whereas 528 Hz alone or a non-sequential order does not. Conclusion: Rein’s preliminary experiments indicate that human DNA is highly sensitive to acoustic energy. While a 528 Hz tone by itself did not alter DNA, the combination and order of Solfeggio frequencies had a notable impact on DNA’s electrical properties. This implies that synergistic or sequential resonances of multiple Solfeggio tones might underlie some reputed “healing” effects. (Note: These findings, shared in 2024, are part of ongoing research and have yet to appear in a peer-reviewed journal; they represent gray literature from a collaboration between researcher Dr. Rein and sound healer J. Goldman.)
  • Sound Healing Using Solfeggio Frequencies (Sonya Joseph – 2019, Master’s Thesis). Frequencies: All six classic Solfeggio frequencies (396, 417, 528, 639, 741, 852 Hz) and extended tones (174, 285, 963 Hz) in context of healing practices. Objective: Provide a comprehensive overview of how Solfeggio frequencies can be used in music therapy to heal physical, emotional, and spiritual trauma; correlate ancient frequency traditions with modern science. Methodology: Academic thesis involving## Literature Reviews, Theses, and Commentary
  • “Sound Healing Using Solfeggio Frequencies” – Sonya Joseph (2019, M.A. Thesis). Frequencies: Comprehensive survey of all Solfeggio frequencies (174–963 Hz) in therapeutic contexts. Objective: To investigate and document how Solfeggio tones and music therapy can facilitate healing of physical, emotional, and even “generational” trauma. Joseph’s graduate research reviews historical and scientific information linking these frequencies to mind-body effects. Methodology: Literature review and theoretical analysis (no primary experiment). She correlated ancient Solfeggio scale concepts with modern neuroscience and psychology, including chakra energy systems and vibrational healing principles. Key Findings: The thesis concluded that Solfeggio frequencies can be “effective tools for universal healing,” helping to transform the brain and relieve mental, emotional, and physical suffering. Joseph noted evidence (from other studies) that each frequency could influence the body (e.g. 528 Hz reducing stress via hormone changes), and she advocated integrating sound frequency therapy with conventional medicine. Conclusion: While largely theoretical, this academic work lent support to Solfeggio-based sound healing, citing that negative emotions may correspond to specific Solfeggio tones and that using these frequencies could enhance well-being.
  • “A Review on the Effects of Chanting and Solfeggio Frequencies on Well-Being” – Xuyu Yang, Fiona F. H. Nah, Fen Lin (2023). Frequencies: Focuses on Solfeggio tones and chanting in general (not tied to one specific frequency). Objective: Summarize existing studies on how chanting practices and Solfeggio frequency music affect brain activity and wellness, and identify gaps for future research. Methodology: Systematic literature review (presented at an HCI conference; published in Lecture Notes in Computer Science). It compiles research across fields (neuroscience, psychology, alternative medicine) related to sound healing. Key Insights: The authors found some preliminary scientific evidence supporting benefits of Solfeggio frequencies (and mantra chanting) for well-being, such as reduced stress and enhanced mood, but overall the topic is under-researched and lacks large clinical trials. They call for more rigorous studies and empirical validation of the effects of these specific frequencies on the mind and body. Conclusion: While anecdotal and small-scale studies abound, there is a need for more scientific investigation into Solfeggio frequency healing to conclusively determine their therapeutic value.
  • “Certain Frequency Music…Possible Effective Healing” (H. Bando, A. Yoshioka, M. Bando, et al., 2023, Commentary). Frequencies: Highlights 528 Hz and others (including 432 Hz, 639 Hz, 852 Hz). Content: A short commentary in the International Journal of Complementary & Alternative Medicine discussing the rise of interest in specific “healing” frequencies. It notes that music tuned to frequencies like 432 Hz, 528 Hz, 639 Hz, 852 Hz has been reported to reduce tension and anxiety, but scientific evidence remains limited. The authors liken the phenomenon to the “Mozart effect” – an intriguing idea needing more proof. They encourage further research to accumulate rigorous evidence, as current support for Solfeggio or 432 Hz benefits is still mostly preliminary. (This serves as a perspective piece underscoring the gap between popular claims and scientific validation.)

Sources: The above entries draw from peer-reviewed journals, academic theses, and other scholarly or professional publications that have investigated Solfeggio frequencies and 432 Hz tuning. Key references are cited inline for each study (using the 【 】notation). These include research articles in medical and scientific journals, conference proceedings and theses, as well as credible summaries of ongoing experiments. Each source is linked for further reading. The compilation spans human clinical trials, animal and cellular experiments, literature reviews, and theoretical works to provide a comprehensive overview of the current knowledge on Solfeggio and 432 Hz frequency research.